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biotinylated e. coli o11  (InvivoGen)


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    InvivoGen biotinylated e. coli o11
    Biotinylated E. Coli O11, supplied by InvivoGen, used in various techniques. Bioz Stars score: 94/100, based on 65 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated e. coli o11/product/InvivoGen
    Average 94 stars, based on 65 article reviews
    biotinylated e. coli o11 - by Bioz Stars, 2026-06
    94/100 stars

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    ( a, b ) Streptavidin pulldown of biotinylated (Bio) LPS from HeLa cells that were primed with IFN-γ and transfected with biotin or Bio-LPS for 5 h. Bio-LPS was detected with a streptavidin-conjugated HRP (horse radish peroxidase). IB, immunoblot. ( c ) Immunofluorescent confocal images in BMDMs that were primed with IFN-γ and transfected with FITC-LPS. DNA was stained by DAPI. Arrows point to colocalizations of FITC-LPS, UBXN1 and caspase-11 in WT, and localization of FITC-LPS in UBXN1 -/- BMDMs. Mock: transfection reagent only. Scale bar: 10 µm. ( d ) The cell death (LDH release) of HeLa cells that were primed with 10 ng/mL of IFN-γ for 12 h and transfected with a CASP4 or GSDMD plasmid for 24 h. Error bar, mean ± S.E.M., N=2 independent experiments. ( e ) Immunoblots of GSDMD in HeLa cells transfected with a FLAG-APEX-CASP4 and/or Myc-UBXN1 plasmid. The cells primed with IFN-γ and transfected with LPS serve as a positive control for GSDMD cleavage. FL, full-length; NT, active N-terminal fragment.

    Journal: bioRxiv

    Article Title: Unanchored ubiquitin chains promote the non-canonical inflammasome via UBXN1

    doi: 10.1101/2024.10.30.621131

    Figure Lengend Snippet: ( a, b ) Streptavidin pulldown of biotinylated (Bio) LPS from HeLa cells that were primed with IFN-γ and transfected with biotin or Bio-LPS for 5 h. Bio-LPS was detected with a streptavidin-conjugated HRP (horse radish peroxidase). IB, immunoblot. ( c ) Immunofluorescent confocal images in BMDMs that were primed with IFN-γ and transfected with FITC-LPS. DNA was stained by DAPI. Arrows point to colocalizations of FITC-LPS, UBXN1 and caspase-11 in WT, and localization of FITC-LPS in UBXN1 -/- BMDMs. Mock: transfection reagent only. Scale bar: 10 µm. ( d ) The cell death (LDH release) of HeLa cells that were primed with 10 ng/mL of IFN-γ for 12 h and transfected with a CASP4 or GSDMD plasmid for 24 h. Error bar, mean ± S.E.M., N=2 independent experiments. ( e ) Immunoblots of GSDMD in HeLa cells transfected with a FLAG-APEX-CASP4 and/or Myc-UBXN1 plasmid. The cells primed with IFN-γ and transfected with LPS serve as a positive control for GSDMD cleavage. FL, full-length; NT, active N-terminal fragment.

    Article Snippet: Biotinylated LPS ( E. coli 0111:B4, Cat# tlrl-lpsbiot) was a product of InvivoGen.

    Techniques: Transfection, Western Blot, Staining, Plasmid Preparation, Positive Control

    Interaction and immunoprecipitation of sLN and CD14. ( A ) Schematic on the co-immunoprecipitation of CD14 and sLN and dot-blot showing enrichment of sLN following pull-down with CD14. ( B ) Schematic demonstrating the competitive co-immunoprecipitation of biotinylated LPS for CD14 in the presence of unlabeled LPS and sLN. Western blot showing the detection of CD14 following the indicated pull-downs. Lanes from left to right: control (CD14 only), LPS-treated, sLN-treated. Original Western blot images are available in .

    Journal: Biomolecules

    Article Title: Short Link N Modulates Inflammasome Activity in Intervertebral Discs Through Interaction with CD14

    doi: 10.3390/biom14101312

    Figure Lengend Snippet: Interaction and immunoprecipitation of sLN and CD14. ( A ) Schematic on the co-immunoprecipitation of CD14 and sLN and dot-blot showing enrichment of sLN following pull-down with CD14. ( B ) Schematic demonstrating the competitive co-immunoprecipitation of biotinylated LPS for CD14 in the presence of unlabeled LPS and sLN. Western blot showing the detection of CD14 following the indicated pull-downs. Lanes from left to right: control (CD14 only), LPS-treated, sLN-treated. Original Western blot images are available in .

    Article Snippet: Competitive IP was performed by loading Avidin-labelled agarose beads (ThermoFisher Scientific; cat# 20219) with 1.0 μg biotinylated LPS (LPS-Biotin) (InvivoGen, San Diego, CA, USA; cat# tlrl-lpsbiot).

    Techniques: Immunoprecipitation, Dot Blot, Western Blot, Control